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HOW TO MAKE MICROSCOPE SLIDES
SECTION CUTTING

HOW TO MAKE MICROSCOPE SLIDES
SECTION CUTTING
BY
DR. MARSH

SECTION-CUTTING

A PRACTICAL GUIDE

to the

PREPARATION AND MOUNTING OF SECTIONS
FOR THE MICROSCOPE

SPECIAL PROMINENCE BEING GIVEN TO THE
SUBJECT OF ANIMAL SECTIONS

BY

DR SYLVESTER MARSH

WITH ILLUSTRATIONS

LONDON
J. & A. CHURCHILL, NEW BURLINGTON STREET

1878 - 128 Pages.

FILE FORMAT - 6.77 MB - .PDF


CONTENTS.

PART I.

Introduction 9
On Cutting Unprepared Vegetable Tissues 10
On Cutting Unprepared Animal Tissues 12
Preparation of Vegetable Tissues 14
Preparation of Animal Tissues 15
Special Methods of Hardening 19
Cutting Hardened Tissues by Hand 19
Microtome 21
Ether Microtome 26
Section Knife 26
Imbedding in Paraffine for Microtome 27
Employment of Microtome 31
Staining Agents 34
Carmine Staining 36
Mounting Media 39
Mounting in Glycerine 41
Uses of Freezing Microtome 47
Employment of Freezing Microtome 48
Logwood Staining 53
Absolute Alcohol 56
Clove Oil 57
Canada Balsam 58
Mounting in Balsam 61
Finishing the Slide 63

PART II.

Special Methods 65
Bone 65
Brain 68
Cartilage 69
Coffee Berry 72
Fat 72
Hair 72
Horn, etc. 73
Intestine 74
Liver 75
Lung 75
Muscle 76
Orange-peel 77
Ovary 77
Porcupine Quill 78
Potato 78
Rush 78
Skin 78
Spinal Cord 81
Sponge 82
Stomach 82
Tongue 83
Vegetable Ivory 83
Wood 83

PREFACE.

IF we glance at any of the numerous magazines
devoted either wholly or in part to the
subject of microscopy, we shall hardly fail to
be struck with the numerous queries relating
to SECTION-CUTTING, which are to be found in
its pages. A simple explanation of this widespread
want is afforded by the fact that the
use of the microscope has at the present day
extended to (and is still rapidly spreading
amongst) vast numbers of students, who, in
many instances, possess neither the leisure nor
the means to refer for information to large and
expensive text-books. Moreover, were they
actually to consult such works, they would
practically fail to obtain the information of
which they are in need, for the coveted instruction
is to be found in those treatises
only in a scattered and fragmentary form no
work with which we are acquainted treating
of the subject in anything like a detailed
manner. To fill this vacuum in the literature
of microscopy the present manualette has been
prepared. Little claim is made to originality,
yet the book is by no means a mere compilation,
but the outcome of long and extensive
personal experience in the cutting and
mounting of microscopical sections. Every
process described has been put to the test of
actual trial, so that its worth may confidently
be depended upon. Many of the little points
insisted upon in the ensuing pages,will doubtless
to the practised microscopist appear
superfluous or even puerile ; but a vivid
recollection of our own early failures and
disappointments assures us that it is just
these very minutiae of detail which will be
found most serviceable in directing and sustaining
the faltering footsteps of the tyro.

ST. HELENS,
September, 1878.

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